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FRANCHISE RESALES

Buy a Bio-One Franchise

Bio-One is a biohazard and crime scene cleanup franchise that handles situations most cleaning companies are not equipped to manage: trauma and crime scenes, unattended deaths, suicides, blood spills, hoarding environments, medical waste, and infectious-disease decontamination. The brand serves individuals and families, property managers, and first responders, and works alongside police departments, hospitals, and victim advocates.

Founded in 2006 and franchising since 2010, Bio-One runs a home-based model with no storefront or retail buildout. Each location is locally owned and operated but backed by national training, safety protocols, and consistent standards, with a network of more than 100 locations across more than 40 states. Bio-One is part of Five Star Franchising, a multi-brand platform that provides shared marketing, technology, and operational support.

The brand operates under a “Help First, Business Second” mission, pairing technical remediation with discreet, compassionate service. Unmarked vehicles and strict confidentiality are part of its standard approach, positioning Bio-One as a service partner rather than a conventional cleaning vendor.

Latest Resales

  • There are currently no Bio-One resales available

Why buy a Bio-One Franchise?

Bio-One operates in an essential, non-discretionary category: biohazard and trauma remediation is needed regardless of the economy, giving the model resilience across cycles. Most jobs are high-ticket with strong margins, and work flows from steady referral relationships with police, hospitals, coroners, property managers, and insurers rather than from discretionary consumer spending.

To buy a Bio-One franchise is to step into a home-based, low-overhead model with no commercial lease, backed by Five Star Franchising’s marketing, technology, and operational infrastructure. The brand’s compassion-led positioning and “Help First, Business Second” mission help it stand out in a category where trust and discretion drive referrals.

Bio-One Franchise Costs & Information

Based on 2024 data reported in the 2025 FDD

  • Brand Profile

    • Year Founded

      2006

    • Franchising Since

      2010

    • New Startup Cost

      $134,645 – $221,095

    • Avg. Resale Price

      Contact us for details

  • Franchise Fees

    • Initial franchise Fee

      $60,000

      for new territories

    • Transfer Fee

      $15,000

      plus any sales commission owed

    • Royalty Fee

      7.5%

      of Gross Revenue

    • Marketing Fee

      2%

      of Gross Sales or $200 min

  • Unit Performance

    • Total Units *

      137

    • New Units Opened *

      23

    • Units Transferred *

      4

    • Avg. Unit revenue *

      $388,926

    * Based on 2024 data reported in the 2025 FDD

Bio-One – Additional  Information

  1. Training & Support

    Initial training covers 40 hours of combined classroom and on-the-job instruction across operations, technical remediation, and customer service, with new owners typically launching within 45 to 60 days. Backed by Five Star Franchising, ongoing support includes purchasing co-ops, digital marketing tools (SEO, PPC, and a professional website), sales and marketing playbooks, online resources, field experience, dedicated franchise consultants, and a collaborative owner network. Resale buyers should confirm with Bio-One what transfer training and system access pass to incoming owners and existing staff.

  2. Ideal Owner

    Bio-One suits service-minded local operators who can build and maintain referral relationships with police, hospitals, coroners, property managers, and victim advocates. No prior cleanup or emergency-response experience is required; the role rewards strong communicators who bring composure and compassion to sensitive situations, manage a small on-call team, and coordinate 24/7 response. Backgrounds in first response, the military, healthcare, sales, or operations fit well, but discretion, reliability, and community focus matter more than industry credentials.

  3. Recognition & Awards

    Bio-One has been ranked in Entrepreneur's Franchise 500 for several consecutive years through the early 2020s, and has appeared on FranServe's Fran-Tastic 500 and the Inc. 5000. It has been featured on A&E's Hoarders and CBS's 60 Minutes and is a member of the FBI National Academy Associates. As a Five Star Franchising brand, it is also backed by a platform recognized by Entrepreneur, Franchise Business Review, and the Inc. 5000. For resale buyers, brand recognition should be weighed alongside the specific territory's local reviews and referral reputation.

  4. Industry / Market

    Bio-One operates in the North American biohazard and crime scene cleanup market, the largest regional share of a category growing at a mid-single-digit annual rate (around 5%) into the early 2030s. Demand is driven by unattended deaths in an aging population, opioid and fentanyl incidents, wider awareness of hoarding, and insurance coverage that often offsets cost. The work is essential, seasonless, and non-discretionary. For resale buyers, established agency and insurer relationships within a territory signal stable local demand.

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